The Rat IFN-γ (Interferon Gamma) ELISA Kit is a highly specialized assay designed for the quantitative detection of IFN-γ levels in rat biological samples. IFN-γ is a critical cytokine involved in various immune responses, particularly in activating macrophages, stimulating natural killer cells, and modulating antigen presentation. This ELISA kit offers exceptional sensitivity and specificity in detecting and measuring IFN-γ concentrations, enabling researchers to accurately quantify this vital cytokine in rat samples.
IFN-γ plays a key role in both innate and adaptive immunity, making it an essential factor in studying immune responses, infections, and immune-related diseases. Manufactured to stringent quality control standards, this ELISA kit provides robust and reliable performance, delivering reproducible results for researchers investigating the role of IFN-γ in immune regulation, inflammation, and host defense mechanisms in rats.
Product Name:
Rat IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
AEES00292
Target:
Rat IFN-γ (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-114
87-100
Average (%)
92
105
93
1:4
Range (%)
99-113
84-100
91-105
Average (%)
106
91
98
1:8
Range (%)
98-111
84-97
98-113
Average (%)
105
91
104
1:16
Range (%)
96-111
81-92
91-103
Average (%)
102
87
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
90-103
98
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.2
267.2
908.9
99.3
278.9
944.1
Standard deviation
6
14.7
48.2
6.1
16.5
34.0
C V (%)
5.49
5.5
5.3
6.14
5.92
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
