Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) CLIA Kit
The Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) CLIA Kit is a highly reliable and accurate tool for measuring IGFBP-3 levels in rat samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, providing researchers with dependable and consistent results for various research applications.IGFBP-3 is a critical protein that regulates the bioavailability and activity of insulin-like growth factors, impacting cell growth, differentiation, and survival. Dysregulation of IGFBP-3 has been linked to various diseases and conditions, including cancer, diabetes, and growth disorders, making it a valuable biomarker for studying these pathologies and exploring potential therapeutic interventions.
With its advanced technology and user-friendly design, the Rat IGFBP-3 CLIA Kit from Assay Genie is a valuable asset for researchers looking to investigate the role of IGFBP-3 in health and disease, offering a precise and efficient solution for measuring IGFBP-3 levels in rat samples.
Product Name:
Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) CLIA Kit
SKU:
RTES00318
Target:
Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGFBP-3. You can calculate the concentration of Rat IGFBP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
87-100
88-104
Average (%)
105
93
95
1:4
Range (%)
100-116
86-100
92-108
Average (%)
107
92
100
1:8
Range (%)
93-107
88-102
95-108
Average (%)
100
96
102
1:16
Range (%)
96-113
103-119
90-101
Average (%)
103
109
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.23
87.81
428.5
47.64
92.3
452.3
Standard deviation
4.17
7.9
47.56
4.03
10.44
36.05
C V (%)
8.47
9.0
11.1
8.46
11.31
7.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGFBP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP-3 in samples. No significant cross-reactivity or interference between Rat IGFBP-3 and analogues was observed.
