Rat IGFBP5 (Insulin Like Growth Factor Binding Protein 5) CLIA Kit
The Rat IGFBP5 (Insulin-Like Growth Factor Binding Protein 5) CLIA Kit is a highly sensitive assay designed for the accurate quantification of IGFBP5 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers high specificity and reproducibility, ensuring reliable results for a variety of research applications.IGFBP5 is an important regulator of insulin-like growth factor (IGF) signaling, controlling the availability and activity of IGFs in the body.
It plays a crucial role in cell growth, differentiation, and apoptosis, making it a key player in various physiological processes and diseases.By accurately measuring IGFBP5 levels, researchers can gain valuable insights into the role of this protein in conditions such as growth disorders, cancer, and metabolic diseases. This kit provides a powerful tool for investigating the mechanisms of IGFBP5 action and developing potential therapeutic interventions.
Product Name:
Rat IGFBP5 (Insulin Like Growth Factor Binding Protein 5) CLIA Kit
SKU:
RTES00312
Target:
Rat IGFBP5 (Insulin Like Growth Factor Binding Protein 5)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGFBP5. You can calculate the concentration of Rat IGFBP5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
97-110
93-107
Average (%)
102
104
100
1:4
Range (%)
97-108
91-105
89-105
Average (%)
102
98
96
1:8
Range (%)
100-116
98-113
103-115
Average (%)
108
104
109
1:16
Range (%)
100-116
94-108
85-98
Average (%)
106
99
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
91-108
98
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.06
151.37
432.91
55.59
166.16
450.67
Standard deviation
6.61
13.15
26.84
7.2
13.11
29.61
C V (%)
12.01
8.69
6.2
12.95
7.89
6.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGFBP5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP5 in samples. No significant cross-reactivity or interference between Rat IGFBP5 and analogues was observed.
