The Rat IgG (Immunoglobulin G) CLIA Kit (RTES00305) from Assay Genie is a reliable and accurate tool for the quantification of IgG levels in rat serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Immunoglobulin G is a critical component of the immune system, playing a crucial role in the body's defense against infections and diseases.
Monitoring IgG levels can provide valuable insights into the immune response and help researchers study various immune-related conditions and treatments.With the Rat IgG CLIA Kit from Assay Genie, researchers can easily and efficiently measure IgG levels in rat samples, making it an essential tool for immunology research and drug development studies. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Rat IgG (Immunoglobulin G) CLIA Kit
SKU:
RTES00305
Target:
Rat IgG (Immunoglobulin G)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IgG. You can calculate the concentration of Rat IgG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
99-115
97-110
Average (%)
95
107
104
1:4
Range (%)
85-96
96-111
89-104
Average (%)
91
102
95
1:8
Range (%)
94-108
93-106
98-112
Average (%)
101
98
105
1:16
Range (%)
97-112
89-105
90-103
Average (%)
105
96
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
98
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.05
67.76
201.64
27.27
66.04
207.23
Standard deviation
2.52
4.96
17.78
3.19
7.25
15.5
C V (%)
10.06
7.32
8.82
11.7
10.98
7.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IgG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgG in samples. No significant cross-reactivity or interference between Rat IgG and analogues was observed.
