The Rat IgM (Immunoglobulin M) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of rat IgM levels in various biological samples. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.IgM is an essential component of the immune system, playing a crucial role in the body's defense against pathogens.
Elevated levels of IgM can indicate an active immune response or infection, making it a valuable biomarker for studying various immune-related conditions and diseases.Whether you are researching immunology, infectious diseases, or autoimmune disorders, the Rat IgM CLIA Kit from Assay Genie is a valuable tool for your laboratory. Order now and unlock the potential of your research!
Product Name:
Rat IgM (Immunoglobulin M) CLIA Kit
SKU:
RTES00306
Target:
Rat IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IgM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IgM. You can calculate the concentration of Rat IgM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
97-112
101-117
Average (%)
93
103
108
1:4
Range (%)
93-110
101-114
98-108
Average (%)
101
107
103
1:8
Range (%)
92-108
88-102
96-112
Average (%)
99
94
103
1:16
Range (%)
90-102
85-97
91-104
Average (%)
96
90
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
96-113
104
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.64
41.04
154.86
21.32
45.02
147.52
Standard deviation
2.08
3.67
11.72
2.53
3.71
9.49
C V (%)
9.61
8.94
7.57
11.87
8.24
6.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgM in samples. No significant cross-reactivity or interference between Rat IgM and analogues was observed.
