The Rat IL-13 ELISA Kit is meticulously crafted to facilitate the quantitative detection of Interleukin-13 (IL-13) levels in a variety of rat biological samples. IL-13 is a pivotal cytokine that exhibits diverse immunoregulatory functions, playing a crucial role in immune responses, inflammation, and allergic reactions. Known for its involvement in modulating the immune system and influencing the pathogenesis of various diseases, IL-13 serves as a key player in regulating allergic inflammation, tissue repair, and host defense mechanisms.
With cutting-edge technology and precision engineering, Assay Genie's Rat IL-13 ELISA Kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit guarantees robust performance and ease of use, making it an indispensable tool for researchers exploring the intricate roles of IL-13 in health and disease.
Product Name:
Rat IL-13 ELISA Kit
SKU:
AEES00455
Target:
Rat IL-13
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-13. You can calculate the concentration of Rat IL-13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
94-110
84-97
Average (%)
94
101
91
1:4
Range (%)
89-101
86-100
89-102
Average (%)
96
92
94
1:8
Range (%)
90-106
85-98
86-98
Average (%)
98
90
92
1:16
Range (%)
93-109
81-97
85-100
Average (%)
100
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.59
85.49
414.83
53.72
92.42
378.61
Standard deviation
2.87
4.27
17.63
3.1
3.99
16.85
C V (%)
5.79
4.99
4.25
5.77
4.32
4.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-13 in samples. No significant cross-reactivity or interference between Rat IL-13 and analogues was observed.
