The Rat IL-13 ELISA Kit is meticulously crafted to facilitate the quantitative detection of Interleukin-13 (IL-13) levels in a variety of rat biological samples. IL-13 is a pivotal cytokine that exhibits diverse immunoregulatory functions, playing a crucial role in immune responses, inflammation, and allergic reactions. Known for its involvement in modulating the immune system and influencing the pathogenesis of various diseases, IL-13 serves as a key player in regulating allergic inflammation, tissue repair, and host defense mechanisms. With cutting-edge technology and precision engineering, Assay Genie's Rat IL-13 ELISA Kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit guarantees robust performance and ease of use, making it an indispensable tool for researchers exploring the intricate roles of IL-13 in health and disease.
Product Name:
Rat IL-13 ELISA Kit
SKU:
AEES00455
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
