Description
Rat IL-16 ELISA Kit
The Rat IL-16 ELISA Kit is expertly crafted for the precise quantification of Interleukin-16 (IL-16) levels in a broad range of rat biological samples. IL-16, a multifunctional cytokine, plays a crucial role in immune modulation, cell recruitment, and inflammatory responses within the body. This cytokine's involvement in various cellular processes underscores its significance as a mediator of immune regulation, cell migration, and inflammatory cascades.
Assay Genie's Rat IL-16 ELISA Kit is engineered with state-of-the-art technology to deliver exceptional sensitivity and specificity, ensuring the accurate and reproducible measurement of IL-16 levels. Manufactured under strict quality control measures, this kit guarantees robust performance and user-friendly operation, making it an indispensable tool for researchers investigating the intricate functions of IL-16 in health and disease.
| Product Name: | Rat IL-16 ELISA Kit |
| Product Code: | AEES00456 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat IL-16 in samples. No significant cross-reactivity or interference between Rat IL-16 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat IL-16 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-16. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-16. You can calculate the concentration of Rat IL-16 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
