The Rat IL-1ra/IL-1F3 ELISA Kit is a specialized assay designed for the precise quantification of Interleukin-1 Receptor Antagonist (IL-1ra/IL-1F3) levels in a variety of rat biological samples. IL-1ra is a crucial anti-inflammatory cytokine that serves as an antagonist to interleukin-1 (IL-1), the pro-inflammatory cytokine.
IL-1ra acts to competitively inhibit the binding of IL-1 to its receptor, thereby modulating inflammatory responses and maintaining immune homeostasis. Its dysregulation is associated with various inflammatory diseases, making accurate measurement of IL-1ra essential for understanding its role in immune modulation and disease pathogenesis. The Rat IL-1ra/IL-1F3 ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, enabling the reliable and reproducible quantification of IL-1ra levels in research samples. Manufactured under stringent quality control standards, this kit ensures robust performance and consistent results, making it an ideal choice for researchers studying immune responses, inflammation, and cytokine regulation.
Product Name:
Rat IL-1ra/IL-1F3 ELISA Kit
SKU:
AEES00450
Target:
Rat IL-1ra/IL-1F3
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1ra/IL-1F3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1ra/IL-1F3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1ra/IL-1F3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1ra/IL-1F3. You can calculate the concentration of Rat IL-1ra/IL-1F3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
97-111
93-104
Average (%)
99
104
99
1:4
Range (%)
92-107
83-93
84-96
Average (%)
98
88
90
1:8
Range (%)
87-100
85-98
86-101
Average (%)
93
92
93
1:16
Range (%)
95-109
81-91
88-99
Average (%)
100
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
258.12
469.42
1787.69
236.74
446.26
1746.27
Standard deviation
14.48
22.63
55.24
14.73
24.81
90.81
C V (%)
5.61
4.82
3.09
6.22
5.56
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-1ra/IL-1F3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Rat IL-1ra/IL-1F3 and analogues was observed.
