The Rat IL-5 ELISA Kit is expertly designed for the precise and quantitative detection of Interleukin-5 (IL-5) levels in diverse rat biological samples. IL-5 is a crucial cytokine known for its involvement in promoting the differentiation, maturation, and activation of various immune cells, especially eosinophils. As a key regulator of the immune response, IL-5 plays a significant role in allergic responses, asthma, and other immune-mediated conditions. Accurate measurement of IL-5 levels is essential for unraveling its specific functions in immune modulation and inflammatory processes.
Assay Genie's Rat IL-5 ELISA Kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance, making it an ideal tool for research into the roles of IL-5 in immune responses and inflammatory disorders. The kit's user-friendly design further enhances its utility, providing researchers with a reliable and efficient solution for cytokine quantification studies.
Product Name:
Rat IL-5 ELISA Kit
SKU:
AEES00452
Target:
Rat IL-5
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-5. You can calculate the concentration of Rat IL-5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
90-106
89-103
Average (%)
103
97
94
1:4
Range (%)
93-107
85-101
85-95
Average (%)
99
92
90
1:8
Range (%)
91-106
80-93
86-98
Average (%)
98
87
91
1:16
Range (%)
90-107
86-96
86-99
Average (%)
98
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.22
135.74
491.76
45.95
134.06
457.13
Standard deviation
2.56
6.81
26.36
2.71
7.74
17.33
C V (%)
5.42
5.02
5.36
5.9
5.77
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-5 in samples. No significant cross-reactivity or interference between Rat IL-5 and analogues was observed.
