Based on the information provided in the URL, the Rat Indoleamine 2,3-Dioxygenase (IDO) ELISA Kit is a powerful tool for accurately measuring levels of IDO in rat samples. This kit is specifically designed for use with rat serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for reliable and reproducible results.IDO is an important enzyme involved in tryptophan metabolism and immune regulation. Its activity has been linked to various physiological and pathological processes, including immune response modulation, inflammation, and cancer progression.
The Rat IDO ELISA Kit provides researchers with a valuable tool for studying the role of IDO in these processes and potentially identifying new therapeutic targets.Overall, the Rat IDO ELISA Kit is a valuable asset for researchers working with rat models and seeking to delve deeper into the complex mechanisms regulated by IDO in various biological contexts. Its accuracy, reliability, and versatility make it an essential resource for advancing our understanding of IDO biology and potentially developing innovative treatment strategies.
Product Name:
Rat Indoleamine-2,3-Dioxygenase (IDO) ELISA Kit
Product Code:
RTDL00522
Size:
96 Assays
Target:
IDO
Synonyms:
CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
