The Rat INS (Insulin) ELISA Kit is a specialized assay designed for the accurate and precise quantification of insulin levels in various biological samples collected from rat models. Insulin is a critical hormone involved in glucose metabolism, cell growth, and energy storage processes, playing a pivotal role in regulating blood sugar levels and overall metabolic homeostasis.
This ELISA kit provides researchers with a reliable tool to quantify insulin concentrations, allowing for the investigation of insulin secretion, metabolism, and its impact on various physiological and pathological conditions. Accurate measurement of insulin is essential for understanding its role in diabetes, metabolic disorders, and other related conditions. Manufactured under strict quality control standards, the Rat INS ELISA Kit ensures exceptional sensitivity and specificity, enabling researchers to obtain reliable and reproducible results. Its robust performance and ease of use make it an excellent choice for studies focused on insulin biology and metabolic research in rat models.
Product Name:
Rat INS (Insulin) ELISA Kit
SKU:
AEES00548
Target:
Rat INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-102
84-97
-
Average (%)
98
92
-
1:4
Range (%)
88-99
95-105
-
Average (%)
91
101
-
1:8
Range (%)
98-104
92-100
-
Average (%)
101
96
-
1:16
Range (%)
96-107
99-110
-
Average (%)
99
103
-
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
94
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
-
-
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
83-97
nan
90.0
nan
nan
nan
Mean (ng/mL)
20
20.0
20.0
20.0
20.0
20.0
Standard deviation
19.26
40.93
195.61
17.58
47.64
176.39
C V (%)
0.98
1.86
8.22
1.05
2.19
7.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.
