The Rat Interleukin-1 Beta (IL1B) ELISA Kit is specifically designed for the precise measurement of IL1B levels in rat serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IL1B is a key pro-inflammatory cytokine that plays a critical role in the regulation of immune responses and inflammation. Dysregulation of IL1B has been implicated in various diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions.
Therefore, this ELISA kit is an invaluable tool for investigating the role of IL1B in disease pathogenesis and for identifying potential therapeutic targets.Overall, the Rat Interleukin-1 Beta (IL1B) ELISA Kit is a reliable and efficient assay for studying the biological functions of IL1B and its implications in disease development, offering researchers a valuable tool for advancing their research in immunology and inflammatory processes.
Product Name:
Rat Interleukin 1 Beta (IL-1b) ELISA Kit
Product Code:
RTDL00552
Size:
96 Assays
Target:
IL1b
Synonyms:
IL1-B, IL1-Beta, IL1F2, IL-1?, Interleukin-1 Family Member 2, Catabolin
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
