The Rat KISS1 (Kisspeptin-1) ELISA Kit is a powerful tool for measuring levels of kisspeptin-1 in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for various research applications.Kisspeptin-1, a neuropeptide encoded by the KISS1 gene, is known for its role in regulating the hypothalamic-pituitary-gonadal axis and reproductive function. Studies have shown that dysregulation of kisspeptin-1 levels is associated with conditions such as infertility, puberty disorders, and reproductive cancers, highlighting the importance of monitoring kisspeptin-1 levels.
By using the Rat KISS1 ELISA Kit, researchers can gain valuable insights into the role of kisspeptin-1 in reproductive physiology and pathology, paving the way for the development of novel diagnostic and therapeutic strategies.
Product Name:
Rat KISS1 (Kisspeptin 1) ELISA Kit
SKU:
RTES01144
Target:
Rat KISS1 (Kisspeptin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KISS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KISS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KISS1. You can calculate the concentration of Rat KISS1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-96
85-97
94-109
Average (%)
90
91
101
1:4
Range (%)
89-103
82-92
89-100
Average (%)
96
88
95
1:8
Range (%)
92-104
86-99
83-95
Average (%)
99
91
90
1:16
Range (%)
91-104
82-96
85-99
Average (%)
98
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
228.82
578.41
2346.06
244.62
578.38
2521.3
Standard deviation
13.41
32.04
79.3
15.78
27.94
92.28
C V (%)
5.86
5.54
3.38
6.45
4.83
3.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat KISS1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
