The Rat LEP (Leptin) ELISA Kit is a highly sensitive assay meticulously designed for the quantitative assessment of Leptin levels in various rat biological samples.
Leptin is a pivotal hormone that regulates energy balance, satiety, and body weight by signaling the brain to reduce appetite and increase energy expenditure. Imbalances in Leptin levels have been associated with obesity, metabolic disorders, and other health conditions, making its accurate measurement essential for research into metabolic mechanisms and potential therapeutic strategies. With exceptional sensitivity and specificity, our ELISA kit ensures precise and reproducible results, enabling researchers to explore the intricate role of Leptin in metabolic homeostasis effectively. Manufactured under stringent quality standards, this kit from Assay Genie provides reliable performance and user-friendly protocols, making it an ideal choice for investigative studies in the field of metabolism and energy regulation in rat models.
Product Name:
Rat LEP (Leptin) ELISA Kit
SKU:
AEES00466
Target:
Rat LEP (Leptin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
88-104
83-97
Average (%)
102
95
90
1:4
Range (%)
98-114
88-101
93-106
Average (%)
106
93
99
1:8
Range (%)
93-110
86-98
96-112
Average (%)
101
91
104
1:16
Range (%)
100-116
84-97
95-107
Average (%)
108
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.5
4.4
0.5
1.5
4.8
Standard deviation
0.03
0.08
0.17
0.03
0.08
0.23
C V (%)
6
5.33
3.86
6.0
5.33
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed.
