The Rat LH (Luteinizing Hormone) CLIA Kit is a highly specific and sensitive assay designed for the quantitative measurement of LH levels in rat serum, plasma, and tissue homogenates. This kit offers reliable and reproducible results, making it an essential tool for studying reproductive health and the endocrine system in rats.Luteinizing hormone is a key hormone involved in the regulation of reproductive function, particularly in female ovulation and male testosterone production. Dysregulation of LH levels can lead to fertility issues, hormonal imbalances, and other reproductive disorders.
This kit provides researchers with a valuable tool for investigating these processes and developing potential treatments.Whether studying reproductive physiology, endocrinology, or animal models of reproductive disorders, the Rat LH CLIA Kit offers a convenient and accurate solution for measuring LH levels in rat samples. Trust Assay Genie for high-quality, reliable assays for your research needs.
Product Name:
Rat LH (Luteinizing Hormone) CLIA Kit
SKU:
RTES00021
Target:
Rat LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
87-104
97-112
Average (%)
97
95
104
1:4
Range (%)
103-115
104-118
98-116
Average (%)
109
110
106
1:8
Range (%)
96-110
89-104
97-114
Average (%)
101
95
104
1:16
Range (%)
99-116
97-112
88-102
Average (%)
106
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
107
EDTA plasma (n=5)
99-110
104
Cell culture media (n=5)
102-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
3.15
7.5
0.94
3.06
6.9
Standard deviation
0.13
0.23
0.72
0.08
0.26
0.55
C V (%)
12.75
7.3
9.6
8.51
8.5
7.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed.
