The Rat LTF (LF) Lactoferrin CLIA Kit is specifically designed for the accurate and reliable detection of lactoferrin levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise results for a variety of research applications.Lactoferrin is an important protein that plays a crucial role in the immune system, as well as in iron metabolism and antimicrobial activity.
Its levels can be indicative of various health conditions, making it a valuable biomarker for studying diseases such as inflammation, infection, and cancer.With its easy-to-use format and excellent performance characteristics, the Rat LTF (LF) Lactoferrin CLIA Kit is an essential tool for researchers looking to explore the role of lactoferrin in health and disease.
Product Name:
Rat LTF/LF (Lactoferrin) CLIA Kit
SKU:
RTES00354
Target:
Rat LTF/LF (Lactoferrin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LF/LTF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LF/LTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LF/LTF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LF/LTF. You can calculate the concentration of Rat LF/LTF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
92-104
97-109
Average (%)
96
98
103
1:4
Range (%)
101-118
96-108
99-111
Average (%)
108
102
105
1:8
Range (%)
103-115
96-109
85-100
Average (%)
109
103
92
1:16
Range (%)
103-118
95-108
93-111
Average (%)
109
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
102-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.07
73.9
382.39
40.07
72.32
399.29
Standard deviation
3.72
5.62
34.57
4.17
8.43
33.18
C V (%)
8.44
7.6
9.04
10.41
11.66
8.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LF/LTF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LF/LTF in samples. No significant cross-reactivity or interference between Rat LF/LTF and analogues was observed.
