The Rat Macrophage-Derived Chemokine (MDC) ELISA Kit is a reliable and accurate tool for detecting MDC levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Macrophage-Derived Chemokine (MDC) is a key protein involved in immune responses, specifically in attracting and activating immune cells like macrophages and T cells.
It plays a critical role in inflammatory processes and immune system regulation, making it a valuable marker for studying immune-related conditions and potential therapies.With the Rat Macrophage-Derived Chemokine (MDC) ELISA Kit, researchers can confidently measure MDC levels and gain valuable insights into the immune response in rat models, advancing our understanding of inflammatory diseases and potential treatment strategies.
Product Name:
Rat Macrophage Derived Chemokine (MDC) ELISA Kit
Product Code:
RTDL00659
Size:
96 Assays
Target:
MDC
Synonyms:
CCL22, ABCD1, DC/B-CK, SCYA22, STCP1, Chemokine C-C-Motif Ligand 22, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22, Stimulated T-Cell Chemotactic Protein 1
Detection Method:
Sandwich
Range:
23.437-1,500pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
