Rat Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
The Rat Macrophage Inflammatory Protein-1 alpha (MIP1A) ELISA Kit is a valuable tool for the accurate measurement of MIP1A levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MIP1A is a key chemokine involved in the regulation of inflammatory responses, playing a crucial role in recruiting immune cells to sites of infection or tissue damage.
It is implicated in a wide range of inflammatory conditions, making it a valuable biomarker for studying immune responses and developing potential therapeutics.Overall, the Rat Macrophage Inflammatory Protein-1 alpha (MIP1A) ELISA Kit provides researchers with a reliable tool for accurately measuring MIP1A levels in rat samples, enabling in-depth investigations into the role of this chemokine in various pathophysiological processes.
Product Name:
Rat Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
Product Code:
RTDL00670
Size:
96 Assays
Target:
MIP1a
Synonyms:
CCL3, MIP1-A, SCYA3, Chemokine C-C-Motif Ligand 3, Small Inducible Cytokine A3, Homologous To Mouse Mip-1a, Tonsillar Lymphocyte LD78 Alpha Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
