Rat MAPtau (Microtubule Associated Protein Tau) CLIA Kit
The Rat MAPTau (Microtubule-Associated Protein Tau) CLIA Kit is specifically designed for the quantitative detection of MAPTau levels in rat samples. This kit offers high sensitivity and specificity, guaranteeing reliable and consistent results for a variety of research applications.MAPTau is a vital protein that plays a crucial role in regulating microtubule assembly and stabilization within neurons. Dysregulation of MAPTau has been implicated in various neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease, making it a valuable biomarker for studying these conditions and exploring potential treatment strategies.
Overall, the Rat MAPTau CLIA Kit is a valuable tool for researchers looking to better understand the role of MAPTau in neurodegenerative disorders and related conditions, providing accurate and precise measurements for insightful research outcomes.
Product Name:
Rat MAPtau (Microtubule Associated Protein Tau) CLIA Kit
SKU:
RTES00537
Target:
Rat MAPtau (Microtubule Associated Protein Tau)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MAPÏ„. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAPÏ„ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAPÏ„, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MAPÏ„. You can calculate the concentration of Rat MAPÏ„ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-107
97-108
Average (%)
92
97
102
1:4
Range (%)
101-117
99-114
89-103
Average (%)
106
107
97
1:8
Range (%)
99-114
92-104
86-100
Average (%)
106
98
91
1:16
Range (%)
94-109
88-102
95-110
Average (%)
101
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
101-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.38
79.95
285.19
38.86
84.51
262.95
Standard deviation
4.64
8.7
29.35
3.92
6.35
29.42
C V (%)
10.95
10.88
10.29
10.09
7.51
11.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MAPÏ„ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MAPÏ„ in samples. No significant cross-reactivity or interference between Rat MAPÏ„ and analogues was observed.
