Rat MAPtau (Microtubule Associated Protein Tau) ELISA Kit
The Rat MAPTAU (Microtubule-Associated Protein Tau) ELISA Kit is specifically designed for the accurate quantification of MAPTAU levels in rat samples such as serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for researchers in various fields.MAPTAU is a critical protein involved in microtubule stabilization and neuronal function, with dysregulation of MAPTAU being linked to neurodegenerative disorders such as Alzheimer's disease.
By measuring MAPTAU levels, researchers can gain insights into the pathogenesis of these conditions and explore potential therapeutic interventions.Overall, the Rat MAPTAU ELISA Kit is an indispensable resource for studying the role of MAPTAU in neurodegenerative diseases and advancing our understanding of neuronal function.
Product Name:
Rat MAPtau (Microtubule Associated Protein Tau) ELISA Kit
SKU:
RTES00784
Target:
Rat MAPtau (Microtubule Associated Protein Tau)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MAPτ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAPτ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAPτ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MAPτ. You can calculate the concentration of Rat MAPτ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
88-104
98-111
Average (%)
95
95
103
1:4
Range (%)
90-102
84-97
88-99
Average (%)
96
89
93
1:8
Range (%)
92-108
84-97
86-99
Average (%)
100
88
92
1:16
Range (%)
87-99
86-97
83-97
Average (%)
94
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.74
266.64
706.17
90.3
245.67
753.07
Standard deviation
4.75
13.87
30.29
6.28
10.34
40.36
C V (%)
5.12
5.2
4.29
6.95
4.21
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MAPÏ„ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MAPÏ„ in samples. No significant cross-reactivity or interference between Rat MAPÏ„ and analogues was observed.
