The Rat/Mau Microalbuminuria CLIA Kit (RTES00020) is a cutting-edge assay designed for the precise measurement of microalbuminuria levels in rat and mouse samples. This kit offers exceptional accuracy and sensitivity, ensuring dependable and consistent results for a variety of research applications. Microalbuminuria is a key indicator of kidney health and is often used as a marker for early detection of kidney disease in animal models. Monitoring microalbuminuria levels can provide valuable insights into the progression of kidney-related conditions, making this kit an essential tool for researchers studying renal function and developing potential treatments.
With its user-friendly protocol and robust performance, the Rat/Mau Microalbuminuria CLIA Kit (RTES00020) is a valuable addition to any laboratory conducting preclinical research on kidney diseases and related disorders in rodent models. Trust Assay Genie for reliable results you can count on.
Product Name:
Rat MAU (Microalbuminuria) CLIA Kit
SKU:
RTES00020
Target:
Rat MAU (Microalbuminuria)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
101-120
89-100
Average (%)
100
109
94
1:4
Range (%)
87-101
95-108
93-107
Average (%)
92
102
99
1:8
Range (%)
96-111
89-102
94-108
Average (%)
101
96
101
1:16
Range (%)
103-115
101-120
93-108
Average (%)
109
109
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.46
7.19
1.08
2.48
7.21
Standard deviation
0.11
0.18
0.59
0.1
0.2
0.55
C V (%)
10.38
7.32
8.21
9.26
8.06
7.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MAU in samples. No significant cross-reactivity or interference between Rat MAU and analogues was observed.
