The Rat Microalbuminuria (MAU) ELISA Kit offers a precise and reliable method for quantitatively detecting microalbuminuria levels in rat biological samples. Microalbuminuria, the presence of small amounts of albumin in urine, serves as an essential marker for early kidney dysfunction, particularly in cardiovascular and renal diseases.
Accurate measurement of microalbuminuria plays a critical role in monitoring and diagnosing renal health, providing valuable insights into the progression of kidney disease and cardiovascular complications in rats. With a focus on sensitivity and specificity, Assay Genie's MAU ELISA Kit ensures accurate and reproducible results, making it an excellent tool for researchers investigating renal function, cardiovascular health, and related pathologies in rat models. The kit is manufactured under stringent quality control standards to guarantee robust performance and user-friendly protocols, enhancing its utility for a wide range of research applications.
Product Name:
Rat MAU (Microalbuminuria) ELISA Kit
SKU:
AEES00300
Target:
Rat MAU (Microalbuminuria)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 μg/mL
Detection range:
1.56-100 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
95-108
98-112
Average (%)
92
102
103
1:4
Range (%)
95-110
82-93
94-110
Average (%)
100
87
100
1:8
Range (%)
94-111
87-99
92-107
Average (%)
102
92
98
1:16
Range (%)
94-106
84-94
91-106
Average (%)
101
89
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.1
8.6
47.9
4.9
8.2
49.2
Standard deviation
0.3
0.4
1.7
0.3
0.4
1.5
C V (%)
5.88
4.65
3.55
6.12
4.88
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MAU in samples. No significant cross-reactivity or interference between Rat MAU and analogues was observed.
