Rat MHC2/AgB2/H-12 (Major Histocompatibility Complex2) CLIA Kit
The Rat MHC2 (AGB2-H-12) CLIA Kit is a cutting-edge assay designed for the accurate measurement of major histocompatibility complex 2 levels in rat samples. This CLIA kit offers high sensitivity and specificity, ensuring precise and reliable results for research purposes.Major histocompatibility complex 2 plays a critical role in the immune response and antigen presentation.
Its levels can be indicative of immune system function and could be potentially useful in studying immune-related diseases and therapies. This CLIA kit is perfect for researchers looking to investigate the role of MHC2 in various physiological and pathological conditions in rat models.
Product Name:
Rat MHC2/AgB2/H-12 (Major Histocompatibility Complex2) CLIA Kit
SKU:
RTES00377
Target:
Rat MHC2/AgB2/H-12 (Major Histocompatibility Complex2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MHCâ…¡/AgBâ…¡/H-1â…¡. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MHCâ…¡/AgBâ…¡/H-1â…¡ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MHCâ…¡/AgBâ…¡/H-1â…¡, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MHCâ…¡/AgBâ…¡/H-1â…¡. You can calculate the concentration of Rat MHCâ…¡/AgBâ…¡/H-1â…¡ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
94-105
85-97
Average (%)
99
99
91
1:4
Range (%)
85-99
100-114
96-111
Average (%)
91
105
104
1:8
Range (%)
99-113
96-113
86-100
Average (%)
107
104
93
1:16
Range (%)
95-109
96-112
104-116
Average (%)
102
103
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.6
9.24
48.23
5.03
8.78
50.51
Standard deviation
0.54
1.02
3.57
0.62
0.96
5.16
C V (%)
11.74
11.04
7.4
12.33
10.93
10.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MHCâ…¡/AgBâ…¡/H-1â…¡ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MHCâ…¡/AgBâ…¡/H-1â…¡ in samples. No significant cross-reactivity or interference between Rat MHCâ…¡/AgBâ…¡/H-1â…¡ and analogues was observed.
