Description
Rat MIP-1beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit
The Rat MIP-1? (Macrophage Inflammatory Protein 1 Beta) ELISA Kit is a specialized assay designed for the precise quantification of MIP-1? levels in rat biological samples.
MIP-1? is a crucial chemokine that regulates inflammation and immune responses by recruiting and activating various immune cells, particularly macrophages and lymphocytes, in diverse physiological and pathological conditions. This ELISA kit provides a reliable and accurate method for researchers to investigate the levels of MIP-1?, shedding light on its roles in immune cell modulation, inflammatory processes, and disease progression. With exceptional sensitivity and specificity, Assay Genie's ELISA kit ensures dependable and reproducible results, making it a valuable tool for researchers studying immunology, inflammation, and related fields. Trust in our kit to deliver robust performance and user-friendly protocols for your research needs.
| Product Name: | Rat MIP-1beta (Macrophage Inflammatory Protein 1 Beta) ELISA Kit |
| Product Code: | AEES00473 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat MIP-1? in samples. No significant cross-reactivity or interference between Rat MIP-1? and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat MIP-1? concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MIP-1?. You can calculate the concentration of Rat MIP-1? in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MIP-1? were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MIP-1? were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 51.50 | 93.80 | 479.00 | 48.90 | 95.60 | 476.80 |
| Standard deviation | 3.60 | 4.80 | 22.50 | 2.80 | 5.50 | 19.10 |
| CV (%) | 6.99 | 5.12 | 4.70 | 5.73 | 5.75 | 4.01 |
Recovery
The recovery of Rat MIP-1? spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 91 |
| EDTA plasma (n=8) | 89-103 | 94 |
| Cell culture media (n=8) | 88-101 | 95 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
