Rat MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
The Rat MIP-3 Alpha (Macrophage Inflammatory Protein-3 Alpha) CLIA Kit is a powerful tool for accurately measuring MIP-3 Alpha levels in rat samples. This kit offers high sensitivity and specificity, providing dependable and consistent results for various research applications.MIP-3 Alpha is a key chemokine that plays a critical role in the recruitment and activation of immune cells, particularly in inflammatory responses. Dysregulation of MIP-3 Alpha has been linked to various diseases, including autoimmune disorders and inflammatory conditions, making it a valuable biomarker for studying these ailments and exploring potential treatments.
With the Rat MIP-3 Alpha CLIA Kit, researchers can confidently analyze MIP-3 Alpha levels in serum, plasma, and tissue culture supernatants, gaining valuable insights into the role of this cytokine in health and disease. Order now to advance your research and uncover new discoveries in the field of immunology and inflammation.
Product Name:
Rat MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
SKU:
RTES00372
Target:
Rat MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-3α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-3α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MIP-3α. You can calculate the concentration of Rat MIP-3α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-105
90-101
99-117
Average (%)
96
95
107
1:4
Range (%)
96-107
96-111
86-96
Average (%)
102
103
91
1:8
Range (%)
99-110
97-114
87-99
Average (%)
104
105
94
1:16
Range (%)
100-118
86-97
89-102
Average (%)
108
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.91
77.49
245.73
23.74
82.08
242.11
Standard deviation
3.04
5.51
20.69
2.92
9.24
22.81
C V (%)
12.2
7.11
8.42
12.3
11.26
9.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MIP-3α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MIP-3α in samples. No significant cross-reactivity or interference between Rat MIP-3α and analogues was observed.
