Rat MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
The Rat Matrix Metalloproteinase 12 (MMP-12) ELISA Kit is a cutting-edge tool meticulously engineered for the precise quantitative measurement of MMP-12 levels in diverse rat biological samples. MMP-12, a member of the matrix metalloproteinase family, plays a crucial role in tissue remodeling, particularly in the degradation of extracellular matrix components. This enzyme is implicated in various physiological and pathological processes, including inflammation, wound healing, and tissue repair. Utilizing this ELISA kit enables researchers to accurately assess the levels of MMP-12, shedding light on its involvement in tissue homeostasis and disease pathogenesis.
The assay offers exceptional sensitivity and specificity, ensuring the generation of consistent and reliable results. Manufactured under strict quality standards, this kit guarantees robust and reproducible performance, providing a user-friendly and efficient solution for investigative studies in areas such as inflammation, fibrosis, and extracellular matrix dynamics.
Product Name:
Rat MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
SKU:
AEES00305
Target:
Rat MMP-12 (Matrix Metalloproteinase 12)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-12. You can calculate the concentration of Rat MMP-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-110
87-101
Average (%)
91
102
92
1:4
Range (%)
91-106
87-100
85-97
Average (%)
98
92
90
1:8
Range (%)
87-99
81-95
82-97
Average (%)
93
87
88
1:16
Range (%)
91-104
83-98
82-95
Average (%)
97
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
246.17
699.83
1945.51
236.26
762.26
2004.72
Standard deviation
14.94
32.82
97.08
13.25
44.59
63.75
C V (%)
6.07
4.69
4.99
5.61
5.85
3.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat MMP-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-12 in samples. No significant cross-reactivity or interference between Rat MMP-12 and analogues was observed.
