The Rat MMP-13 (Matrix Metalloproteinase-13) CLIA Kit is specifically designed for the accurate detection of MMP-13 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.MMP-13, also known as collagenase-3, is a key enzyme involved in the breakdown of extracellular matrix components. It plays a critical role in tissue remodeling, wound healing, and various disease processes including cancer, arthritis, and cardiovascular diseases.
By measuring MMP-13 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially develop new therapeutic strategies.Overall, the Rat MMP-13 CLIA Kit is an invaluable tool for studying the role of MMP-13 in various biological processes and diseases, allowing researchers to advance their understanding and potentially identify new treatment options.
Product Name:
Rat MMP-13 (Matrix Metalloproteinase 13) CLIA Kit
SKU:
RTES00027
Target:
Rat MMP-13 (Matrix Metalloproteinase 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-13. You can calculate the concentration of Rat MMP-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
103-115
99-111
Average (%)
91
109
104
1:4
Range (%)
92-107
88-104
98-111
Average (%)
99
96
103
1:8
Range (%)
94-108
85-98
98-113
Average (%)
99
91
105
1:16
Range (%)
99-112
92-105
102-114
Average (%)
106
97
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
87-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
207.7
592.05
1471.71
227.4
580.39
1357.45
Standard deviation
21.7
41.44
138.34
23.29
48.98
143.75
C V (%)
10.45
7.0
9.4
10.24
8.44
10.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-13 in samples. No significant cross-reactivity or interference between Rat MMP-13 and analogues was observed.
