The Rat MMP-3 (Matrix Metalloproteinase-3) CLIA Kit is a powerful tool for detecting MMP-3 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Matrix Metalloproteinase-3, also known as Stromelysin-1, is an enzyme involved in tissue remodeling and inflammatory processes. Elevated levels of MMP-3 have been implicated in various disease states, including arthritis, cancer, and cardiovascular diseases.
By accurately measuring MMP-3 levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.Overall, the Rat MMP-3 CLIA Kit is a valuable asset for researchers studying matrix metalloproteinases and their role in disease pathogenesis. Its high-quality components and precise assay methodology make it a reliable choice for advancing scientific knowledge in the field.
Product Name:
Rat MMP-3 (Matrix Metalloproteinase 3) CLIA Kit
SKU:
RTES00382
Target:
Rat MMP-3 (Matrix Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-3. You can calculate the concentration of Rat MMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
98-113
89-103
Average (%)
104
104
95
1:4
Range (%)
96-113
87-98
93-107
Average (%)
103
92
100
1:8
Range (%)
90-102
100-114
86-98
Average (%)
95
108
91
1:16
Range (%)
100-117
87-99
99-117
Average (%)
108
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.9
269.6
875.66
106.24
263.31
870.52
Standard deviation
12.45
25.5
55.95
13.11
22.51
52.67
C V (%)
11.43
9.46
6.39
12.34
8.55
6.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.
