The Rat MMP-9 (Matrix Metalloproteinase-9) CLIA Kit is specially designed for the precise measurement of MMP-9 levels in rat serum, plasma, and tissue homogenate samples. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent choice for various research applications.MMP-9 is a key enzyme involved in extracellular matrix remodeling, playing a critical role in tissue development, wound healing, and inflammatory processes.
Dysregulation of MMP-9 has been implicated in various pathological conditions, including cancer, arthritis, and cardiovascular diseases, highlighting its importance as a biomarker for disease progression and potential therapeutic targets.Overall, the Rat MMP-9 CLIA Kit offers researchers a reliable tool for investigating the role of MMP-9 in disease physiology and exploring novel treatment strategies.
Product Name:
Rat MMP-9 (Matrix Metalloproteinase 9) CLIA Kit
SKU:
RTES00387
Target:
Rat MMP-9 (Matrix Metalloproteinase 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-9. You can calculate the concentration of Rat MMP-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
96-107
99-118
Average (%)
99
101
108
1:4
Range (%)
88-100
101-116
102-121
Average (%)
94
109
110
1:8
Range (%)
93-107
88-104
89-102
Average (%)
100
95
95
1:16
Range (%)
90-102
96-109
90-101
Average (%)
95
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.15
4.13
0.48
1.08
4.14
Standard deviation
0.06
0.09
0.46
0.04
0.12
0.47
C V (%)
11.32
7.83
11.14
8.33
11.11
11.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-9 in samples. No significant cross-reactivity or interference between Rat MMP-9 and analogues was observed.
