The Rat Melatonin (MT) ELISA Kit is a specialized assay designed for the quantitative measurement of Melatonin levels in various biological samples. Melatonin, a hormone secreted by the pineal gland in response to darkness, is a key regulator of the circadian rhythm and plays important roles in sleep-wake cycles, antioxidant activity, and various physiological processes. By accurately quantifying Melatonin levels, researchers can gain insights into the circadian system and its impact on various biological functions and health conditions.
Utilizing this ELISA kit enables researchers to precisely measure Melatonin concentrations in rat samples, providing valuable information for studies related to sleep disorders, chronobiology, and neuroendocrinology. The assay ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for research exploring the role of Melatonin in circadian biology and related fields.
Product Name:
Rat MT (Melatonin) ELISA Kit
SKU:
AEES00303
Target:
Rat MT (Melatonin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
88-99
97-109
Average (%)
103
94
102
1:4
Range (%)
89-100
89-104
97-108
Average (%)
94
97
103
1:8
Range (%)
87-98
88-100
98-110
Average (%)
92
94
104
1:16
Range (%)
88-100
91-102
96-109
Average (%)
93
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
102
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.5
95.0
358.5
52.5
88.9
364.1
Standard deviation
2.7
4.3
18.6
2.7
4.4
19.3
C V (%)
5.24
4.53
5.19
5.14
4.95
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
