Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit
The Rat N-Acetyl-Beta-D-Glucosaminidase (NAGase) ELISA Kit is a valuable tool for detecting levels of NAGase in rat serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and consistent results, making it an excellent choice for various research applications.NAGase is an enzyme that plays a crucial role in the breakdown of glycosaminoglycans and is associated with various pathological conditions such as lysosomal storage disorders and renal diseases.
By accurately measuring NAGase levels, researchers can gain valuable insights into disease mechanisms and potential treatment strategies.This ELISA kit provides researchers with a reliable and efficient method for studying NAGase levels in rat samples, offering a comprehensive understanding of its biological functions and implications in disease pathology. Trust in the Rat NAGase ELISA Kit for robust and accurate quantification of NAGase levels in your research studies.
Product Name:
Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
91-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
