The Rat NCAD (Neural Cadherin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NCAD levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an ideal tool for research applications related to neural cadherin.NCAD, also known as neural cadherin, is a key protein involved in cell adhesion and communication in the nervous system. It plays a critical role in neuronal development, synaptic function, and plasticity.
Dysregulation of NCAD expression has been linked to various neurological disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat NCAD CLIA Kit provides researchers with a valuable tool for studying the role of neural cadherin in neurodevelopmental and neurodegenerative disorders, as well as in exploring potential treatment options in these areas.
Product Name:
Rat NCAD (Neural Cadherin) CLIA Kit
SKU:
RTES00076
Target:
Rat NCAD (Neural Cadherin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NCAD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NCAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NCAD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NCAD. You can calculate the concentration of Rat NCAD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
91-105
95-113
Average (%)
109
98
103
1:4
Range (%)
84-99
90-105
94-106
Average (%)
91
96
100
1:8
Range (%)
96-110
96-111
99-114
Average (%)
104
104
105
1:16
Range (%)
99-115
88-99
83-99
Average (%)
107
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.66
200.79
971.59
97.43
204.76
877.81
Standard deviation
9.89
19.7
105.61
8.98
18.78
99.19
C V (%)
10.45
9.81
10.87
9.22
9.17
11.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NCAD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NCAD in samples. No significant cross-reactivity or interference between Rat NCAD and analogues was observed.
