Rat NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit
The Rat NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit is specifically designed to quantitatively assess the levels of NFKB-p65 in various rat biological samples.
NFKB-p65, also known as RelA, is a key subunit of the nuclear factor kappa B (NF-κB) transcription factor complex involved in regulating genes related to inflammation, immunity, cell survival, and various disease processes. Its pivotal role in orchestrating cellular responses to stimuli makes it a significant target for investigating inflammatory pathways, immune modulation, and disease pathogenesis. Accurate measurement of NFKB-p65 levels through this ELISA kit provides researchers with valuable insights into the activation and regulation of NF-κB signaling, offering a deeper understanding of its involvement in various physiological and pathological conditions. By elucidating the intricate mechanisms governed by NFKB-p65, researchers can unravel its significance in immune responses, inflammatory disorders, and potential therapeutic interventions targeted at NF-κB pathways. The Rat NFKB-p65 ELISA Kit by Assay Genie assures exceptional sensitivity and specificity, guaranteeing dependable and reproducible results. With robust performance and user-friendly protocols, this kit emerges as an excellent choice for research endeavors seeking to decipher the complex landscape of NF-κB signaling and its implications in health and disease.
Product Name:
Rat NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit
SKU:
AEES00498
Target:
Rat NFKB-p65 (Nuclear Factor Kappa B p65)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p65. You can calculate the concentration of Rat NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-112
95-107
Average (%)
103
103
100
1:4
Range (%)
88-103
87-102
84-97
Average (%)
95
93
89
1:8
Range (%)
90-103
85-97
83-96
Average (%)
97
90
89
1:16
Range (%)
92-105
88-99
88-99
Average (%)
97
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
247.16
793.15
2350.95
248.82
741.4
2123.84
Standard deviation
13.77
35.06
123.19
12.69
32.03
93.02
C V (%)
5.57
4.42
5.24
5.1
4.32
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p65 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NFKB-p65 in samples. No significant cross-reactivity or interference between Rat NFKB-p65 and analogues was observed.
