Description
Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
The Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit is a specialized assay tailored for the precise quantitative detection of NGAL levels in diverse biological samples from rats. NGAL, also known as Lipocalin-2, is a versatile protein involved in various cellular functions, prominently recognized for its role in inflammation, immune response modulation, and kidney injury biomarker status. NGAL is particularly associated with diverse physiological and pathological conditions, making it an indispensable target for investigative studies. This ELISA kit empowers researchers to effectively measure NGAL levels in rat samples, shedding light on its involvement in inflammatory processes, renal health, and potential diagnostic applications in various diseases.
With accurate quantification of NGAL, researchers can enhance their understanding of its significance in pathophysiology and explore its implications as a viable biomarker in clinical settings. The Rat NGAL ELISA Kit by Assay Genie is meticulously developed to offer outstanding sensitivity and specificity, ensuring accurate and reproducible outcomes. With stringent quality control measures in place, this kit delivers robust performance and user-friendly protocols, making it an optimal choice for cutting-edge research applications aiming to dissect the intricacies of NGAL in health and disease.
| Product Name: | Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit |
| Product Code: | AEES00496 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 62.50-4000 pg/mL |
| Sensitivity: | 37.50 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat NGAL in samples. No significant cross-reactivity or interference between Rat NGAL and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat NGAL concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NGAL. You can calculate the concentration of Rat NGAL in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
