The Rat NGB (Neuroglobin) CLIA Kit is specifically designed for the precise measurement of neuroglobin levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Neuroglobin is a vital protein known for its role in protecting neurons and promoting cell survival in conditions such as stroke, traumatic brain injury, and neurodegenerative diseases.
By detecting and quantifying neuroglobin levels, researchers can gain valuable insights into the mechanisms of these conditions and potential therapeutic interventions.In conclusion, the Rat NGB CLIA Kit is an indispensable tool for studying the role of neuroglobin in neurological disorders and advancing our understanding of neuroprotection mechanisms.
Product Name:
Rat NGB (Neuroglobin) CLIA Kit
SKU:
RTES00402
Target:
Rat NGB (Neuroglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NGB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NGB. You can calculate the concentration of Rat NGB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
89-101
90-102
Average (%)
106
96
97
1:4
Range (%)
102-119
95-106
101-116
Average (%)
109
100
109
1:8
Range (%)
87-99
91-107
95-107
Average (%)
93
98
100
1:16
Range (%)
88-100
93-105
98-109
Average (%)
95
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-116
108
EDTA plasma (n=5)
102-115
107
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.88
60.46
242.09
21.77
57.43
241.87
Standard deviation
3.01
5.37
21.62
2.05
6.43
16.28
C V (%)
12.6
8.88
8.93
9.42
11.2
6.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NGB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NGB in samples. No significant cross-reactivity or interference between Rat NGB and analogues was observed.
