The Rat NGF (Nerve Growth Factor) CLIA Kit is a cutting-edge tool designed for the precise measurement of NGF levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.NGF is a vital protein that plays a key role in promoting the growth and survival of nerve cells. It is essential for the development and maintenance of the nervous system and is implicated in various neurological disorders.
As a critical biomarker, NGF holds great promise for advancing research in neurobiology and potentially developing new therapeutic interventions.With its advanced technology and reliability, the Rat NGF CLIA Kit is a valuable asset for researchers seeking to explore the intricate mechanisms of nerve growth and potential interventions for neurological conditions.
Product Name:
Rat NGF (Nerve Growth Factor) CLIA Kit
SKU:
RTES00401
Target:
Rat NGF (Nerve Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NGF. You can calculate the concentration of Rat NGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-101
85-96
93-108
Average (%)
96
90
101
1:4
Range (%)
101-114
92-102
85-97
Average (%)
108
97
91
1:8
Range (%)
96-107
93-111
97-114
Average (%)
101
101
105
1:16
Range (%)
85-100
93-107
95-110
Average (%)
91
98
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.32
103.86
292.54
46.68
97.66
265.52
Standard deviation
4.82
8.87
18.17
4.73
8.5
28.7
C V (%)
11.13
8.54
6.21
10.13
8.7
10.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed.
