The Rat NGF (Nerve Growth Factor) ELISA Kit is a specialized assay designed for the quantitative detection of NGF levels in various rat biological samples. NGF is a critical neurotrophic factor known for its role in promoting the growth, survival, and differentiation of neurons, making it essential for the development and maintenance of the nervous system. Abnormal NGF levels have been implicated in various neurological conditions, including neurodegenerative diseases, neural injuries, and pain disorders, highlighting the significance of accurate NGF measurement in research and clinical studies. Accurate quantification of NGF through this ELISA kit enables researchers to investigate the neurotrophic effects of NGF, study neuronal pathophysiology, and explore potential therapeutic interventions targeting NGF signaling pathways.
By measuring NGF levels in rat samples, researchers can gain insights into the role of this growth factor in neural health and disease progression. The Rat NGF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under rigorous quality control standards, this kit delivers reliable performance and ease of use, making it an excellent choice for neuroscience research and drug discovery studies focusing on NGF biology.
Product Name:
Rat NGF (Nerve Growth Factor) ELISA Kit
SKU:
AEES00492
Target:
Rat NGF (Nerve Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NGF. You can calculate the concentration of Rat NGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-102
89-101
96-111
Average (%)
93
96
103
1:4
Range (%)
88-104
81-93
84-95
Average (%)
95
87
89
1:8
Range (%)
89-99
87-98
84-96
Average (%)
94
92
89
1:16
Range (%)
90-101
86-101
87-98
Average (%)
96
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
90-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
133.12
309.15
1103.7
127.82
305.03
1172.82
Standard deviation
8.52
17.78
53.86
6.62
12.57
56.76
C V (%)
6.4
5.75
4.88
5.18
4.12
4.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed.
