The Rat NRG-1 (Neuregulin-1) CLIA Kit is specifically designed for the precise measurement of NRG-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NRG-1, also known as Neuregulin-1, is a critical protein involved in cell communication and signal transduction. It plays a key role in various physiological processes, including neural development, heart function, and cancer progression.
As a pivotal biomarker, NRG-1 holds significance in studying conditions such as schizophrenia, heart disease, and cancer, making this kit valuable for research aimed at understanding these conditions and developing potential treatments.For more information on the Rat NRG-1 (Neuregulin-1) CLIA Kit, please visit: www.assaygenie.com/rat-nrg-1-neuregulin-1-clia-kit-rtes00477/
Product Name:
Rat NRG-1 (Neuregulin 1) CLIA Kit
SKU:
RTES00477
Target:
Rat NRG-1 (Neuregulin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NRG-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NRG-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NRG-1. You can calculate the concentration of Rat NRG-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-119
90-104
93-107
Average (%)
110
98
101
1:4
Range (%)
86-100
94-108
93-106
Average (%)
93
100
100
1:8
Range (%)
89-102
102-115
101-114
Average (%)
95
108
107
1:16
Range (%)
94-105
92-108
92-105
Average (%)
100
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
413.09
699.86
2979.78
447.02
718.06
2766.05
Standard deviation
37.22
66.21
285.76
40.14
51.05
310.63
C V (%)
9.01
9.46
9.59
8.98
7.11
11.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NRG-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NRG-1 in samples. No significant cross-reactivity or interference between Rat NRG-1 and analogues was observed.
