The Rat NSE (Neuron-Specific Enolase) CLIA Kit is a powerful tool for researchers looking to study neuronal health and function in rats. This kit is specifically designed for the accurate detection of NSE levels in rat samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an essential resource for a wide range of neurobiology research applications.NSE is an important biomarker for neuronal damage and is often used to monitor the health of neurons in conditions such as traumatic brain injury, stroke, and neurodegenerative diseases.
By measuring NSE levels, researchers can gain valuable insights into the status of neuronal cells and potential therapeutic interventions. With the Rat NSE CLIA Kit, researchers can confidently study neurodegeneration and explore novel treatment strategies for neurological disorders.
Product Name:
Rat NSE (Neuron Specific Enolase) CLIA Kit
SKU:
RTES00036
Target:
Rat NSE (Neuron Specific Enolase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NSE. You can calculate the concentration of Rat NSE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
101-120
86-100
Average (%)
100
110
91
1:4
Range (%)
99-113
94-105
91-103
Average (%)
106
99
96
1:8
Range (%)
103-118
100-113
90-105
Average (%)
108
107
98
1:16
Range (%)
104-116
99-114
86-99
Average (%)
110
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
101
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.38
386.63
1439.49
194.05
401.03
1531.01
Standard deviation
22.02
37.5
140.78
23.73
46.92
123.71
C V (%)
11.88
9.7
9.78
12.23
11.7
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NSE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed.
