The Rat Neuron-Specific Enolase (NSE) ELISA Kit is a state-of-the-art assay meticulously designed to quantitatively detect NSE levels in various rat biological samples. Neuron-Specific Enolase, a key neuronal marker, serves as a diagnostic biomarker for neuronal injury or disease, making it a valuable indicator for neuro-related research and clinical applications.
This cutting-edge ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are crucial for understanding neuronal health and function. Manufactured under stringent quality control standards, this kit delivers robust performance, making it an invaluable tool for investigating neuronal processes and disorders. Easy-to-use and reliable, this ELISA kit is an excellent choice for researchers seeking high-quality data in the field of neuroscience.
Product Name:
Rat NSE (Neuron Specific Enolase) ELISA Kit
SKU:
AEES00310
Target:
Rat NSE (Neuron Specific Enolase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NSE. You can calculate the concentration of Rat NSE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
95-109
86-98
Average (%)
97
102
91
1:4
Range (%)
94-110
86-99
96-108
Average (%)
102
93
102
1:8
Range (%)
96-110
87-99
96-110
Average (%)
101
94
104
1:16
Range (%)
96-108
82-94
97-112
Average (%)
102
87
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.2
9.1
1.2
2.3
10.0
Standard deviation
0.1
0.1
0.3
0.1
0.1
0.4
C V (%)
9.09
4.55
3.3
8.33
4.35
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NSE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed.
