Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
The Rat NT-pro-ANP (N-terminal pro-atrial natriuretic peptide) ELISA Kit is specifically designed for the accurate measurement of NT-pro-ANP levels in rat serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NT-pro-ANP is a key biomarker associated with cardiac function and plays a critical role in maintaining fluid balance and blood pressure regulation.
Elevated levels of NT-pro-ANP have been linked to conditions such as heart failure, hypertension, and renal dysfunction, making this kit essential for studying these diseases and potential therapeutic interventions.With its reliable performance and easy-to-use format, the Rat NT-pro-ANP ELISA Kit is a valuable tool for researchers seeking to investigate the role of NT-pro-ANP in cardiovascular health and disease.
Product Name:
Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
SKU:
RTES00989
Target:
Rat NT-Pro-ANP (N-Terminal Pro-Atrial Natriuretic Peptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proANP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proANP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NT-proANP. You can calculate the concentration of Rat NT-proANP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
90-101
91-103
Average (%)
94
96
97
1:4
Range (%)
91-107
80-91
86-100
Average (%)
99
86
91
1:8
Range (%)
88-102
84-97
82-95
Average (%)
94
89
89
1:16
Range (%)
89-101
82-94
88-100
Average (%)
94
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.21
9.72
1.04
2.29
9.26
Standard deviation
0.06
0.13
0.51
0.06
0.13
0.33
C V (%)
6.06
5.88
5.25
5.77
5.68
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NT-proANP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NT-proANP in samples. No significant cross-reactivity or interference between Rat NT-proANP and analogues was observed.
