Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit
The Rat Nuclear Factor erythroid-derived 2-like protein 2 (NFE2L2) ELISA Kit is a powerful tool for the quantitative measurement of NFE2L2 levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for researchers working in a variety of fields.NFE2L2 is a key transcription factor involved in the regulation of antioxidant response elements, playing a critical role in cellular defense against oxidative stress.
Dysregulation of NFE2L2 has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By studying NFE2L2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Rat NFE2L2 ELISA Kit is a valuable tool for studying the role of NFE2L2 in health and disease, providing researchers with a comprehensive platform for accurate and precise quantification of this important protein.
Product Name:
Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
