The Rat Osteonectin CLIA Kit is specifically designed to accurately measure the levels of osteonectin in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Osteonectin is a key protein involved in bone remodeling and mineralization, playing a crucial role in skeletal development and maintenance.
Abnormal levels of osteonectin have been linked to various bone disorders, making it a valuable biomarker for studying bone-related diseases and potential therapeutic interventions.With its reliable performance and wide applicability, the Rat Osteonectin CLIA Kit is a valuable tool for researchers focused on understanding the complex mechanisms of bone metabolism and seeking new treatments for bone disorders.
Product Name:
Rat ON (Osteonectin) CLIA Kit
SKU:
RTES00421
Target:
Rat ON (Osteonectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ON, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ON. You can calculate the concentration of Rat ON in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
96-111
83-98
Average (%)
101
103
90
1:4
Range (%)
98-113
88-102
101-113
Average (%)
106
94
106
1:8
Range (%)
95-107
86-99
100-115
Average (%)
101
92
109
1:16
Range (%)
94-109
92-105
96-108
Average (%)
100
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
101-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.39
68.57
308.25
33.26
69.64
289.09
Standard deviation
3.86
7.17
20.41
2.94
6.43
32.7
C V (%)
10.61
10.46
6.62
8.84
9.23
11.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ON concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ON in samples. No significant cross-reactivity or interference between Rat ON and analogues was observed.
