Based on the information provided in the URL, here is a product description:The Rat OPN (Osteopontin) CLIA Kit is a highly reliable and accurate assay designed for the detection of osteopontin levels in rat samples. This kit is specially optimized for use with rat serum, plasma, and cell culture supernatants, providing researchers with a valuable tool for studying osteopontin levels in various experimental settings.Osteopontin is a key protein involved in bone metabolism, inflammation, and tissue remodeling. It plays a critical role in processes such as wound healing, immune response, and tumor progression, making it an important target for research in fields such as osteology, immunology, and oncology.
With its high sensitivity and specificity, the Rat OPN CLIA Kit offers researchers a dependable method for quantifying osteopontin levels in rat samples, facilitating the discovery of new insights into the role of osteopontin in health and disease. Whether investigating bone disorders, studying immune responses, or exploring cancer biology, this kit provides a valuable tool for advancing scientific knowledge and potential therapeutic development.
Product Name:
Rat OPN (Osteopontin) CLIA Kit
SKU:
RTES00422
Target:
Rat OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OPN. You can calculate the concentration of Rat OPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
90-103
86-99
Average (%)
98
96
91
1:4
Range (%)
94-110
102-117
87-100
Average (%)
100
109
94
1:8
Range (%)
85-95
95-110
92-108
Average (%)
90
103
99
1:16
Range (%)
91-108
104-118
102-116
Average (%)
99
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
94
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.75
6.81
0.92
2.76
6.83
Standard deviation
0.12
0.29
0.67
0.09
0.31
0.6
C V (%)
12.5
10.55
9.84
9.78
11.23
8.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed.
