Rat OxLDL (Oxidized Lowdensity Lipoprotein) CLIA Kit
The Rat OXLDL (Oxidized Low-Density Lipoprotein) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of oxidized LDL levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Oxidized LDL is a key player in atherosclerosis and cardiovascular diseases, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
This kit is an essential tool for researchers looking to better understand the role of oxidized LDL in disease progression and treatment strategies.Overall, the Rat OXLDL CLIA Kit offers researchers a powerful tool for investigating the impact of oxidized LDL on various health conditions and developing new approaches for diagnosis and treatment.
Product Name:
Rat OxLDL (Oxidized Lowdensity Lipoprotein) CLIA Kit
SKU:
RTES00425
Target:
Rat OxLDL (Oxidized Lowdensity Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
95-110
97-111
Average (%)
101
101
102
1:4
Range (%)
91-103
84-97
99-113
Average (%)
98
90
106
1:8
Range (%)
100-116
90-104
90-103
Average (%)
107
97
96
1:16
Range (%)
94-105
89-106
101-115
Average (%)
99
96
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
325.82
878.82
2224.1
340.64
948.59
2443.52
Standard deviation
30.11
100.98
240.43
37.13
92.58
259.99
C V (%)
9.24
11.49
10.81
10.9
9.76
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat OxLDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed.
