The Rat PCIII (Procollagen III) CLIA Kit is specifically designed for the precise and accurate detection of Procollagen III levels in rat samples including serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for various research applications.Procollagen III is a key protein involved in collagen synthesis, crucial for maintaining the structural integrity of connective tissues such as skin, tendons, and joints.
Abnormal levels of Procollagen III have been associated with various diseases and conditions, highlighting its importance as a biomarker for studying and potentially treating these ailments.Order your Rat PCIII (Procollagen III) CLIA Kit today and unlock new possibilities in your research endeavors.
Product Name:
Rat PCIII (Procollagen III) CLIA Kit
SKU:
RTES00606
Target:
Rat PCIII (Procollagen III)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PCâ…¢. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCâ…¢ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCâ…¢, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PCâ…¢. You can calculate the concentration of Rat PCâ…¢ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
90-104
90-101
Average (%)
102
96
95
1:4
Range (%)
98-109
93-104
91-104
Average (%)
104
98
97
1:8
Range (%)
102-114
100-115
88-104
Average (%)
108
107
95
1:16
Range (%)
87-101
97-113
100-114
Average (%)
93
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
100-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.17
209.85
868.02
94.52
210.2
910.43
Standard deviation
9.46
16.22
58.68
11.88
21.99
79.57
C V (%)
9.08
7.73
6.76
12.57
10.46
8.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PCâ…¢ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCâ…¢ in samples. No significant cross-reactivity or interference between Rat PCâ…¢ and analogues was observed.
