Rat PDGF-C (Platelet Derived Growth Factor C) CLIA Kit
The Rat PDGF-C (Platelet-Derived Growth Factor-C) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PDGF-C levels in rat samples. This kit is ideal for researchers studying the role of PDGF-C in various biological processes, including cell growth, wound healing, and tissue remodeling.PDGF-C is a key growth factor involved in cell proliferation and migration, playing a crucial role in angiogenesis and tissue repair. Dysregulation of PDGF-C has been implicated in diseases such as cancer and fibrosis, making it an important biomarker for understanding disease progression and developing targeted therapies.
With its reliable performance and ease of use, the Rat PDGF-C CLIA Kit from AssayGenie is an essential tool for researchers investigating the role of PDGF-C in physiological and pathological processes. Get accurate and reproducible results with this advanced assay kit.
Product Name:
Rat PDGF-C (Platelet Derived Growth Factor C) CLIA Kit
SKU:
RTES00450
Target:
Rat PDGF-C (Platelet Derived Growth Factor C)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGF-C. You can calculate the concentration of Rat PDGF-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
99-114
98-114
Average (%)
107
106
105
1:4
Range (%)
91-105
96-113
86-97
Average (%)
98
104
91
1:8
Range (%)
99-115
101-112
89-104
Average (%)
106
107
96
1:16
Range (%)
99-113
85-98
100-115
Average (%)
105
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.56
73.83
212.22
25.25
66.48
198.07
Standard deviation
2.16
7.64
16.66
2.51
5.32
19.77
C V (%)
8.45
10.35
7.85
9.94
8.0
9.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGF-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-C in samples. No significant cross-reactivity or interference between Rat PDGF-C and analogues was observed.
