The Rat PGF (Placental Growth Factor) ELISA Kit is a specialized assay designed for the quantitative detection of Placental Growth Factor levels in rat biological samples. Placental Growth Factor, also known as PGF, is a key angiogenic factor involved in various physiological processes, particularly in promoting blood vessel growth and vascular development. It plays a crucial role in angiogenesis, vascular remodeling, and tissue regeneration, with implications in embryonic development, organogenesis, and pathological conditions such as ischemic diseases and cancer. Accurate measurement of Placental Growth Factor concentrations using this ELISA kit provides valuable insights into angiogenic processes, vascular health, and the pathophysiology of conditions characterized by abnormal blood vessel formation.
Researchers can utilize this kit to investigate the role of PGF in diverse biological contexts, including wound healing, cardiovascular diseases, and cancer progression, among others. The Rat PGF ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With robust performance and user-friendly protocols, this kit is a reliable tool for research studies aiming to elucidate the intricate mechanisms of angiogenesis and vascular biology in both health and disease.
Product Name:
Rat PGF (Placental Growth Factor) ELISA Kit
SKU:
AEES00508
Target:
Rat PGF (Placental Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PGF. You can calculate the concentration of Rat PGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
95-112
93-106
Average (%)
101
103
98
1:4
Range (%)
93-105
84-97
80-93
Average (%)
99
89
87
1:8
Range (%)
94-106
82-93
88-101
Average (%)
99
88
94
1:16
Range (%)
91-103
86-100
86-97
Average (%)
97
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.51
75.11
187.38
26.6
81.13
201.63
Standard deviation
1.69
4.27
7.46
1.85
4.84
7.04
C V (%)
6.9
5.68
3.98
6.95
5.97
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed.
