The Rat PHB (Prohibitin) CLIA Kit is a powerful tool for the quantitative measurement of prohibitin levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers a high level of accuracy and precision, allowing researchers to confidently analyze and compare prohibitin levels in different experimental conditions.Prohibitin is a multifunctional protein that plays a key role in various cellular processes, including cell cycle regulation, apoptosis, and mitochondrial function. Dysregulation of prohibitin has been implicated in a range of diseases, including cancer, metabolic disorders, and neurodegenerative conditions, highlighting its importance as a potential therapeutic target.
With its reliable performance and user-friendly format, the Rat PHB CLIA Kit is an essential tool for studying prohibitin biology and its implications in disease pathology. Researchers can trust this kit to deliver consistent and reproducible results, advancing our understanding of prohibitin function and its potential as a therapeutic target.
Product Name:
Rat PHB (Prohibitin) CLIA Kit
SKU:
RTES00468
Target:
Rat PHB (Prohibitin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PHB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PHB. You can calculate the concentration of Rat PHB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
100-116
83-96
Average (%)
110
106
90
1:4
Range (%)
96-112
99-115
96-109
Average (%)
104
106
103
1:8
Range (%)
100-120
98-112
84-97
Average (%)
109
104
90
1:16
Range (%)
102-117
100-112
99-115
Average (%)
108
106
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
99-115
108
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.77
597.39
1982.63
232.54
587.19
2114.2
Standard deviation
26.81
44.33
136.6
23.58
50.73
201.48
C V (%)
12.54
7.42
6.89
10.14
8.64
9.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PHB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PHB in samples. No significant cross-reactivity or interference between Rat PHB and analogues was observed.
